Title of article :
Modulated anisotropy fluorescence for quantitative determination of carbaryl and benomyl
Author/Authors :
D??az، نويسنده , , A.Navas and Garc??a S?nchez، نويسنده , , F. and L?pez Guerrero، نويسنده , , M.M.، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2003
Pages :
6
From page :
629
To page :
634
Abstract :
Two individual components in mixtures have been resolved by frequency domain fluorescence technique by measuring the observable quantities which characterize the anisotropy decay; differential anisotropy phase and modulated anisotropy ratio (MAR), which in turn are related to the rotational correlation time. The method presented here is capable of directly resolving binaries mixtures of fluorophores on the basis of differences in their rotational diffusion rates. Our results demonstrate that modulation anisotropy ratio measurements can be used for quantitative determination of small analytes, carbaryl and benomyl, having identical or nearly identical fluorescence spectra. This methodology can be applied with good results when the fluorophores have a suitable MAR difference.
Keywords :
Phase-modulation fluorescence , Dynamic anisotropy , small molecules , carbaryl , Benomyl
Journal title :
Talanta
Serial Year :
2003
Journal title :
Talanta
Record number :
1636672
Link To Document :
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