Author/Authors :
Fujimoto، نويسنده , , Kenzo and Matsuda، نويسنده , , Shigeo and Hayashi، نويسنده , , Masayuki and Saito، نويسنده , , Isao، نويسنده ,
Abstract :
An efficient method for the synthesis of circular oligodeoxyribonucleotides (ODNs) using reversible template-directed photoligation via 5-vinyldeoxycytidine (VC) is described. It is shown that the 5′-end VC of ODN reversibly reacts by photoirraditation with thymine at its own 3′-end in the presence of a single-stranded template via triple helix formation to produce circular DNA. Thermodynamic analysis indicated that the stability of triple helix is remarkably enhanced by the vinyl substituent of VC.
