Author/Authors :
Liu، نويسنده , , Ying and Jiang، نويسنده , , Yanxia and Song، نويسنده , , Wenbo and Lu، نويسنده , , Nan and Zou، نويسنده , , Mingzhu and Xu، نويسنده , , Hongding and Yu، نويسنده , , Zhenxiang، نويسنده ,
Abstract :
A method for the determination of the major serotonin metabolite — 5-hydroxyindole-3-acetic acid (5-HIAA) in human gastric juice by cyclic voltammetry was described. The measurement conditions were investigated. The potential window was chosen from +0.1 to +0.9 V, the supporting electrolyte was 0.025 M PBS solution (pH 2.0). The method allowed determination in the concentration range from 2.0 ×10−7 to 2.0 ×10 −5 M and a detection limit of 80 nM. When samples of gastric juice were analyzed with the method, we obtained the mean content of 5-HIAA in the gastric juice. Meanwhile, interference from other ions and substances were examined. The experimental results indicate that the method for the determination of gastric juice samples is successful.
