Electrochemical studies of rutin interacting with hemoglobin and determination of hemoglobin

Electrochemical investigation of the interaction of rutin (Rt) with hemoglobin (Hb) at a glassy carbon electrode is reported for the first time. With the addition of hemoglobin in a rutin solution, both the reduction and oxidation currents decrease with few changes in the peak potentials. The reaction of rutin with hemoglobin forms a nonelectroactive supramolecular complex Hb–Rt. The equilibrium constant for complex is calculated to be 3.4×106. A satisfactory result has been obtained for the determination of hemoglobin in clinical blood samples.