Title of article
Interaction between organophosphorous hydrolase and paraoxon studied by surface chemistry in situ at air–water interface
Author/Authors
Mello، نويسنده , , Sarita V and Coutures، نويسنده , , Cyril and Leblanc، نويسنده , , Roger M and Cheng، نويسنده , , Tu-Chen and Rastogi، نويسنده , , Vipin K and DeFrank، نويسنده , , Joseph J، نويسنده ,
Issue Information
ماهنامه با شماره پیاپی سال 2001
Pages
7
From page
881
To page
887
Abstract
Subphase conditions have been optimized to obtain stable organophosphorous hydrolase (OPH-EC 3.1.8.1) as Langmuir films. The Langmuir film was characterized by surface pressure and surface potential-area isotherms and UV-Vis spectroscopy in situ. The interaction of an organophosphorous compound, namely Paraoxon, with the OPH film was investigated for various surface pressures. The stability of the monolayer and the evidence of the enzyme activity at air–water interface support the use of enzyme LB films as biosensor.
Keywords
Organophosphorous hydrolase , paraoxon , Langmuir fims
Journal title
Talanta
Serial Year
2001
Journal title
Talanta
Record number
1641930
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