Author/Authors :
Mello، نويسنده , , Sarita V and Coutures، نويسنده , , Cyril and Leblanc، نويسنده , , Roger M and Cheng، نويسنده , , Tu-Chen and Rastogi، نويسنده , , Vipin K and DeFrank، نويسنده , , Joseph J، نويسنده ,
Abstract :
Subphase conditions have been optimized to obtain stable organophosphorous hydrolase (OPH-EC 3.1.8.1) as Langmuir films. The Langmuir film was characterized by surface pressure and surface potential-area isotherms and UV-Vis spectroscopy in situ. The interaction of an organophosphorous compound, namely Paraoxon, with the OPH film was investigated for various surface pressures. The stability of the monolayer and the evidence of the enzyme activity at air–water interface support the use of enzyme LB films as biosensor.
