Amperometric sensor for glucose and hypoxanthine based on a PdIrO2 modified electrode by a co-crosslinking bienzymic system

A glassy carbon electrode (GCE) modified with Pd/IrO2 provides excellent electrocatalytic oxidation of hydrogen peroxide. Glucose oxidase (GOD) and xanthine oxidase (XOD) were co-immobilized on the modified electrode with a thin film Nafion coated on the enzyme layer to form a glucose (Glu)/hypoxanthine (Hx) sensor, without interference from electroactive species such as ascorbic acid (AA) and uric acid (UA). Its response was evaluated with respect to the enzyme amount on the electrode, pH and temperature of the electrolyte. The prepared bienzymic biosensor, used as the detector of HPLC gave a detection limit of 1.0×10−6 mol l−1 Glu and 2.0×10−7 mol l−1 Hx (Hx) with a linear concentration range of 5.0×10−6–2.5×10−3 mol l−1 and 1.0×10−6–5.0×10−4 mol l−1, respectively. Coupled with microdialysis, it was used to monitor the concentrations of Glu and Hx in rat brain.