Author/Authors :
Sadraei، H. نويسنده , , Ghanadian، M. نويسنده Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, I.R.Iran . , , Asghari، G. نويسنده Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. , , Madadi، E. نويسنده Department of Pharmacology & Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. , , Madadi، E. نويسنده Department of Pharmacology & Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. ,
Abstract :
Isovanillin and isoacetovanillon are two components found in P. spinosa Decne.exBoiss extract with no previously reported effect on ileum contractions. Spasmolytic effect of isovanillin and isoacetovanillon were examined on response to electrical field stimulation (EFS), acetylcholine (ACh) and 5-HT in strips of rat ileum. Longitudinal ileum strips were set up in an organ bath containing oxygenated Tyrodeʹs solution. All strips that was contracted in response to EFS, acetylcholine or 5-HT showed relaxation in the presence of isovanillin (5-320 µg/ml), or isoacetovanillon (5-320 µg/ml). Isovanillin and isoacetovanillon inhibited the response to 5-HT with IC50 values of 356?50µM and 622?110µM respectively. They reduced the response to EFS without significantly affecting the acetylcholine response. P. spinosa extract (5-160 µg/ml) in a concentration dependent manner reduced the response to 5-HT, acetylcholine and EFS. This study demonstrated that isovanillin and isoacetovanillon are relaxant of ileum contractions induced by 5-HT and EFS and they have contribution to the relaxant effect of P. spinosa extract but other components are responsible for the inhibition of acetylcholine by the extract.
