Chemiluminescence flow-through sensor for ofloxacin using solid-phase PbO2 as an oxidant

A novel chemiluminscence (CL) flow-through sensor for ofloxacin is described. It was based on the sensitizing effect of ofloxacin on the CL oxidation of sulfite by PbO2 in H2SO4 media. By a very simply means, the solid-phase PbO2 was immobilized inside of the CL flow cell as CL oxidant. The column of solid PbO2 could be reused about 400 times during a period of 50 h. The calibration graph is linear in the range 0.2–10 μg ml−1 with a detection limit of 7.8×10−8 g ml−1 (S/N=3). This method has been successfully applied to determine ofloxacin in pharmaceutical preparation.