Title of article
Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces
Author/Authors
Brown، نويسنده , , Rachel and Smith، نويسنده , , W.Ewen and Graham، نويسنده , , Duncan، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2001
Pages
4
From page
2197
To page
2200
Abstract
A method for the addition of a benzotriazole moiety to the 5′-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.
Keywords
Phosphoramidites , solid-phase synthesis , SERS , Benzotriazole , HPLC , Nucleic acids
Journal title
Tetrahedron Letters
Serial Year
2001
Journal title
Tetrahedron Letters
Record number
1643800
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