Title of article
Synthesis and properties of 2-azidodeoxyadenosine and its incorporation into oligodeoxynucleotides
Author/Authors
Wada، نويسنده , , Takeshi and Mochizuki، نويسنده , , Akira and Higashiya، نويسنده , , Seiichiro and Tsuruoka، نويسنده , , Hiroyuki and Kawahara، نويسنده , , Shun-ichi and Ishikawa، نويسنده , , Masahide and Sekine، نويسنده , , Mitsuo، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2001
Pages
5
From page
9215
To page
9219
Abstract
2-Azidodeoxyadenosine (7) was conveniently synthesized from deoxyguanosine (2) by use of a combined reagent of TMSN3–BuONO. The structure of the tautomer of the azido derivative was determined by 1H NMR. Reaction of 7 with iPr2NP(OEt)2 gave an intermediate 10 of the Staudinger reaction. Incorporation of 7 into a DNA 13mer resulted in a significant decrease of the Tm value of the DNA duplex upon hybridization with the complementary strand. The thermal stability was discussed based on the hydrogen bond energy and electrostatic repulsion.
Journal title
Tetrahedron Letters
Serial Year
2001
Journal title
Tetrahedron Letters
Record number
1648921
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