Double use of microwaves in fatty acid preparation for elaidic acid determination as phenacyl ester using high-performance liquid chromatography in Brazilian fat products

Brazilian samples of fat products like margarines, butters and chocolates were prepared to reversed-phase high-performance liquid chromatography analysis, using a domestic microwave oven for fat hydrolysis and derivatization with phenacyl chloride. The total reaction time was 6 min, recoveries were above 94%. Total reproducibility for whole procedure were 3.5% R.S.D. for elaidic acid (n = 3), the total chromatography analysis was 25 min.