Author/Authors :
Wolff، نويسنده , , Murielle and Seemann، نويسنده , , Myriam and Grosdemange-Billiard، نويسنده , , Catherine and Tritsch، نويسنده , , Denis and Campos، نويسنده , , Narciso and Rodr??guez-Concepci?n، نويسنده , , Manuel and Boronat، نويسنده , , Albert and Rohmer، نويسنده , , Michel، نويسنده ,
Abstract :
2-C-Methyl-d-erythritol cyclodiphosphate is converted into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate by a cell-free system from an Escherichia coli strain overexpressing the gcpE gene. The latter diphosphate, representing probably the last intermediate in the MEP pathway for isoprenoid biosynthesis, was identified by comparison with reference material obtained by chemical synthesis.
Keywords :
Biosynthesis , 2-C-methyl-d-erythritol 2 , gcpE , 4-cyclodiphosphate , LytB , Isoprenoids , 2-C-methyl-d-erythritol 4-phosphate
