Photofermentation of malate for biohydrogen production— A modeling approach

A kinetic model for photofermentative biohydrogen production is developed in this study to predict the dynamics of the process. The proposed model contains 17 parameters to describe cell growth, substrate consumption, and hydrogen evolution as well as inhibition of the process by biomass, light intensity, and substrate. Batch experimental results from the literature were used to calibrate and validate the model with malic acid as a model substrate, using Rhodobacter sphaeroides as a model biomass. Temporal hydrogen evolution and cell growth predicted by the proposed model agreed well with the experimentally measured data obtained from four literature reports, with statistically significant correlation coefficients exceeding 0.9. Based on sensitivity analysis performed with the validated model, only six of the 17 parameters were found to be significant. Model simulations indicated that the range of optimal light intensity for maximum hydrogen yield from malate by R. sphaeroides was 150–250 W/m2.