Application of monofluorophosphate/alkaline phosphatase system in flow injection analysis

Monofluorophosphate was found to be a specific substrate for alkaline phosphatase (EC 3.1.3.1) forming novel biosensing schemes with potentiometric detection. Several utilities of this substrate/enzyme system in analytical chemistry will be demonstrated. The system is useful for direct detection of enzymes and substrates as well as for indirect determination of enzyme inhibitors and cofactors using common potentiometric instrumentation. The analytical values of reported biosensing schemes are significantly improved by their implementation into flow injection analysis. This paper presents recent developments in this area and suggests important prospects for further investigations and applications.