Author/Authors :
Piro، نويسنده , , B. and Zhang، نويسنده , , Q.D. and Reisberg، نويسنده , , Digvir S. Jayas •
Noel D. G. White، نويسنده , , V. and Dang، نويسنده , , L.A. and Duc، نويسنده , , H.T. and Pham، نويسنده , , M.C.، نويسنده ,
Abstract :
A system device using multifunctional conjugated copolymer poly(5-hydroxy-1,4-naphthoquinone-co-hydroxy-2-thioacetic acid-1,4-naphthoquinone) acting both as immobilizing and transducing element for reagentless immunosensor has been constructed. Its functionality was evaluated in an antigen–antibody interaction model using ovalbumin–anti-ovalbumin. It was shown that the system specifically detects via electrochemical signal the antigen–antibody immune interaction in a reagentless context. Comparison to the conventional ELISA technique relevant to performance and sensitivity was presented.
Keywords :
conjugated polymer , Electrochemical immunosensor , Reagentless detection , Antibody–antigen affinity , ELISA assays
