Title of article :
H2O2-sensitive quantum dots for the label-free detection of glucose
Author/Authors :
Hu، نويسنده , , Mei and Tian، نويسنده , , Jing and Lu، نويسنده , , Hao-Ting and Weng، نويسنده , , Li-Xing and Wang، نويسنده , , Lian-Hui، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2010
Pages :
6
From page :
997
To page :
1002
Abstract :
A novel label-free detection system based on CdTe/CdS quantum dots (QDs) was designed for the direct measurement of glucose. Herein we demonstrated that the photoluminescence (PL) of CdTe/CdS QDs was sensitive to hydrogen peroxide (H2O2). With d-glucose as a substrate, H2O2 that intensively quenched the QDs PL can be produced via the catalysis of glucose oxidase (GOx). Experimental results showed that the decrease of the QDs PL was proportional to the concentration of glucose within the range of 1.8 μM to 1 mM with the detection limit of 1.8 μM under the optimized experimental conditions. In addition, the QD-based label-free glucose sensing platform was adapted to 96-well plates for fluorescent assay, enhancing the capabilities and conveniences of this detection platform. An excellent response to the concentrations of glucose was found within the range of 2–30 mM. Glucose in blood and urine samples was effectively detected via this strategy. The comparison with commercialized glucose meter indicated that this proposed glucose assay system is not only simple, sensitive, but also reliable and suitable for practical application. The high sensitivity, versatility, portability, high-throughput and low cost of this glucose sensor implied its potential in point-of-care clinical diagnose of diabetes and other fields.
Keywords :
Photoluminescence , Hydrogen peroxide , Glucose , Glucose oxidase , Quantum dots , Sensor
Journal title :
Talanta
Serial Year :
2010
Journal title :
Talanta
Record number :
1660469
Link To Document :
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