Author/Authors :
Leroux، نويسنده , , Fanny and Liebgott، نويسنده , , Pierre-Pol and Cournac، نويسنده , , Laurent and Richaud، نويسنده , , Pierre and Kpebe، نويسنده , , Arlette and Burlat، نويسنده , , Bénedicte and Guigliarelli، نويسنده , , Bruno and Bertrand، نويسنده , , Patrick and Léger، نويسنده , , Christophe and Rousset، نويسنده , , Marc and Dementin، نويسنده , , Sébastien، نويسنده ,
Abstract :
Reproducing the naturally occurring O2-tolerant hydrogenases is a potential strategy to make the oxygen sensitive enzymes, produced by organisms of biotechnological interest, more resistant. The search for resistance “hotspots” that could be transposed into sensitive hydrogenases is underway. Here, we replaced two residues (Y77 and V78) of the oxygen sensitive [NiFe] hydrogenase from Desulfovibrio fructosovorans with Gly and with Cys, respectively, to copy the active site pocket of the resistant membrane-bound [NiFe] enzyme from Ralstonia eutropha and we examined how this affected oxygen sensitivity. The results are discussed in the light of a short review of the recent results dealing with the reactivity of hydrogenases towards oxygen.
