Novel hybrid organic–inorganic monolithic column containing mesoporous nanoparticles for capillary electrochromatography

The rod-shaped SBA–15–C18 mesoporous nanoparticles were incorporated into hybrid organic–inorganic monolithic column with aminopropyl moiety to develop novel stationary phases with mixing mechanism of reverse phase and ion exchange. Experimental conditions including dispersion pattern, nanoparticles percentage were optimized for simple and stable column preparation. The monolithic columns were evaluated with mixture of organic acids in capillary electrochromatography (CEC) mode and the column efficiency reaches 280, 000 plates/m. The results indicate that the column containing nanoparticles enhances both selectivity and column efficiency due to high specific surface area of nanoparticles and mixing separation mechanism.