Author/Authors :
Rajecky، نويسنده , , Michal and Slaninova، نويسنده , , Iva and Mokrisova، نويسنده , , Petra and Urbanova، نويسنده , , Jana and Palkovsky، نويسنده , , Martin and Taborska، نويسنده , , Eva and Taborsky، نويسنده , , Petr، نويسنده ,
Abstract :
Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new utilization for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by near-UV light. Besides quantification of DNA (LOD=6 ng ml−1) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter into the living cell through the cell membrane.
Keywords :
Chelirubine , intercalation , alkaloid , fluorescence , DNA
