Electrosynthesis of a new enzyme-modified electrode for the amperometric detection of glucose

The electrooxidation of a new aminonaphthalene compound modified with a carboxylic acid function, 1-(5-aminonaphthyl)ethanoic acid (ANEA) was studied, with a view to binding glucose oxidase (GOD) covalently. Its electrooxidation leads to thin films (PANEA) on bare glassy carbon (GC) platinum (Pt) electrodes and also on prepolymerized aminonaphthoquinone (PANQ) films. Polymer-modified bilayer electrodes Pt/PANQ/PANEA were then constructed. GOD was covalently bound onto these electrodes, leading to a Pt/PANQ/PANEA/GOD glucose sensor. The amperometric response to glucose was monitored in aerated and deaerated conditions. The results show very efficient covalent binding of GOD and good enzyme activity on the electrode surface.