Title of article :
Microbial community analysis of mixed anaerobic microflora in suspended sludge of ASBR producing hydrogen from palm oil mill effluent
Author/Authors :
Badiei، نويسنده , , Marzieh and Jahim، نويسنده , , Jamaliah Md and Anuar، نويسنده , , Nurina and Sheikh Abdullah، نويسنده , , Siti Rozaimah and Su، نويسنده , , Lim Swee and Kamaruzzaman، نويسنده , , Mohd Aidil، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
8
From page :
3169
To page :
3176
Abstract :
This study investigated the microbial community of an anaerobic sequencing batch reactor (ASBR) operating at mesophilic temperature under varying hydraulic retention times (HRTs) for evaluating optimal hydrogen production conditions, using palm oil mill effluent (POME) as substrate. POME sludge enriched by heat treatment with hydrogen-producing bacteria was used as inoculum and acclimated with the POME. The microbial community was determined by first isolating cultivable bacteria at each operating HRT and then using polymerase chain reaction (PCR). The PCR products were sequenced and sequence identification was performed using the BLAST algorithm and Genbank database. The findings revealed that about 50% of the isolates present were members of the genus Streptococcus, about 30% were Lactobacillus species and around 20% were identified as species of genus Clostridium. Scanning electron microscopy (SEM) analysis also confirmed the presence of spherical and rod-shaped microbial morphologies in the sludge samples of bioreactor during prolonged cultivation.
Keywords :
Palm oil mill effluent (POME) , biohydrogen , Microbial community , Anaerobic sequencing batch reactor (ASBR)
Journal title :
International Journal of Hydrogen Energy
Serial Year :
2012
Journal title :
International Journal of Hydrogen Energy
Record number :
1669777
Link To Document :
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