Title of article :
Antibody mediated fluorescence enhancement of nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC°)
Author/Authors :
Sellrie، نويسنده , , Frank and Lenz، نويسنده , , Christine and Andersson، نويسنده , , Anika and Wilhelmsson، نويسنده , , L. Marcus and Schenk، نويسنده , , Jِrg A.، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2014
Abstract :
We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC°). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA.
Keywords :
Homogeneous immunoassay , 3-Diaza-2-oxophenoxazine (tC°) , monoclonal antibody , Fluorescent nucleoside analogue , Fluorescence enhancement , 1 , Hypsochromic shift
