Functionalized anion exchange stationary phase for separation of anionic compounds

Synthesis of the multilayered stationary phases containing quaternary ammonium functional groups on the silica support was described. Bonded phases were characterized by elemental analysis, solid state 13C NMR spectroscopy and chromatographic methods. The surface of silica support was coated with different number of polymeric layers formed by condensation polymerization of primary amine (methylamine) with diepoxide (1,4-butanedioldiglycidyl ether). A series of stationary phases with different number of polymerized layers were tested. Separation of an inorganic anions sample (F−, Cl−, NO2−, Br−, NO3−) and nucleotides was performed.