Title of article :
Detection of proteins on membranes and in microchannels using copper staining combined with scanning electrochemical microscopy
Author/Authors :
Carano، نويسنده , , Maurizio and Lion، نويسنده , , Niels and Girault، نويسنده , , Hubert H.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Abstract :
Scanning electrochemical microscopy (SECM) and copper staining are combined to achieve visualisation of proteins on surfaces. Proteins are adsorbed on a polyvinylidene fluoride (PVDF) membrane or on a polyethylene terephthalate (PET) surface and stained using a standard protocol involving copper salts. Salts are then reduced to copper and detected by SECM with ferrocene methanol as a redox mediator in aqueous solution. During the SECM scan, the potential is held at a value at which the oxidation of the redox mediator occurs and a positive feedback current is detected when scanning over copper clusters. A negative feedback is observed elsewhere. This method enables unspecific protein adsorption mapping on polymeric membranes and into microchannels without any requirement of enzymatic activity or affinity to a labelled secondary reporter.
Keywords :
Protein detection , SECM , Copper staining , MEMBRANE , PVDF , Scanning electrochemical microscopy , polyvinylidene fluoride
Journal title :
Journal of Electroanalytical Chemistry
Journal title :
Journal of Electroanalytical Chemistry