Simple hemoglobin–gold nanoparticles modified electrode for the amperometric detection of acrylamide

In this work we propose the design of an electrochemical setup (hemoglobin–gold-nanoparticles–ITO glass), used for the determination of a hemoglobin adduct (acrylamide). Acrylamide is neurotoxin and potential carcinogen, formed mainly in high temperatures processed food that contains large quantity of starch (French fries, baked bread). Acrylamide forms hemoglobin adducts, and our sensing device is based on this property, by passivating the hemoglobin-modified electrode by the addition of low concentrations of acrylamide. The sensitivity to acrylamide better as 0.1 μM makes this electrochemical cell configuration suitable for the detection of acrylamide in food products.