A novel l-lactate sensor based on enzyme electrode modified with ZnO nanoparticles and multiwall carbon nanotubes

A highly sensitive and stable l-lactate sensor based on the synergic action of multi-wall carbon nanotube (MWCNTs) and ZnO nanoparticles has been developed. The unique sandwich-like layer structure (PDDA/LOD/ZnO/MWCNTs) provides a favorable microenvironment to keep the bioactivity of LOD and prevent enzyme molecule leakage. Therefore, the proposed l-lactate biosensor exhibited good analytical performances including high sensitivity and selectivity with satisfactory stability to amperometric determination of l-lactate. The results indicated that the proposed PDDA/LOD/ZnO/MWCNTs film was an attractive matrix for the immobilization of LOD enzymes to fabricate biosensors.