Title of article :
A ToF-SIMS and XPS study of protein adsorption and cell attachment across PEG-like plasma polymer films with lateral compositional gradients
Author/Authors :
Menzies، نويسنده , , Donna J. and Jasieniak، نويسنده , , Marek and Griesser، نويسنده , , Hans J. and Forsythe، نويسنده , , John S. and Johnson، نويسنده , , Graham and McFarland، نويسنده , , Gail A. and Muir، نويسنده , , Benjamin W.، نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2012
Pages :
10
From page :
1798
To page :
1807
Abstract :
In this work we report a detailed X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) study of poly(ethylene glycol) PEG-like chemical gradients deposited via plasma enhanced chemical vapour deposition (PECVD) at two different load powers using diethylene glycol dimethyl ether (DG) as a monomer. Principal component analysis (PCA) was applied to the ToF-SIMS data both before and after protein adsorption on the plasma polymer thin films. Results of the PCA loadings indicated a higher content of hydrocarbon fragments across the higher load power gradient, which adsorbed higher amounts of proteins. Gradients deposited at a lower load power retained a higher degree of monomer like functionality as did the central region directly underneath the knife edge electrode. Analysis of the adsorption of serum proteins (human serum albumin and fetal bovine serum) was monitored across the gradient films and increased with decreasing ether (PEG-like) film chemistries. The effect of protein incubation time on the levels adsorbed fetal bovine serum on the plasma polymer films was critical, with significantly more protein adsorbing after 24 hour incubation times on both gradient films. The attachment of HeLa cells on the gradients appeared to be dictated not only by the surface chemistry, but also by the adsorption of serum proteins. XPS analysis revealed that at surface ether concentrations of less than 70% in the gradient films, significant increases in protein and cell attachment were observed.
Keywords :
Gradient , TOF-SIMS , Cell attachment , Diglyme , Plasma polymer , PEG , protein adsorption
Journal title :
Surface Science
Serial Year :
2012
Journal title :
Surface Science
Record number :
1687101
Link To Document :
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