Title of article
Three-step purification of bacterially expressed human single-chain Fv antibodies for clinical applications
Author/Authors
Laroche-Traineau، نويسنده , , J and Clofent-Sanchez، نويسنده , , G and Santarelli، نويسنده , , X، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2000
Pages
11
From page
107
To page
117
Abstract
We have obtained a cell line which secretes a human monoclonal IgM (B7) reacting with the myosin heavy chain of human heart. We have constructed single-chain fragments (scFv) of B7. The scFv may be useful for the imaging of myocardial necrosis after myocarditis, cardiac drug toxicosis or graft rejection. The aim of our work was to purify the scFv for immunoscintigraphy. We describe several purification steps including immobilized metal affinity chromatography (IMAC), anti-c-myc monoclonal antibody affinity chromatography, size-exclusion chromatography with SuperdexR 75 HR 10/30 and ion-exchange chromatography (mini Q TM 30Q).
Keywords
Single-chain Fv antibodies
Journal title
Journal of Chromatography B Biomedical Sciences and Applications
Serial Year
2000
Journal title
Journal of Chromatography B Biomedical Sciences and Applications
Record number
1702746
Link To Document