Phytic acid as an efficient low-molecular-mass displacer for anion-exchange displacement chromatography of proteins

Phytic acid, inositol-hexaphosphoric acid, molecular mass 650, a low-molecular-mass compound, has been identified as a nearly ideal displacer in anion-exchange displacement chromatography for the concentration and purification of model protein mixture. The concentration of low-molecular-mass displacer is a very important parameter for successful separation by displacement chromatography. Displacer concentration influences the formation of the isotachic train and the yield and recovery of the displacement chromatographic process. There is an optimum displacer concentration in which the yield and recovery are highest.