Determination of aloenin, barbaloin and isobarbaloin in Aloe species by micellar electrokinetic chromatography

Aloenin, barbaloin and isobarbaloin in JP Aloe11JP Aloe means a product based on the Japanese Pharmacopeia and is the dried juice of Aloe ferox Miller or its hybrid with Aloe africana Miller or Aloe spicata Baker, which are the so-called Cape Aloes. barbadensis (Aloe vera) and Aloe arborescens Miller var. natalensis Berger (Aloe arborescens Miller) were determined by micellar electrokinetic chromatography (MEKC) with 50 mM sodium dodecyl sulfate. Aloenin, barbaloin and isobarbaloin were well separated by MEKC and as little as 5.5 pg/11 nl of the three compounds could be detected. The determination took around 14 min.