Author/Authors :
Na، نويسنده , , Dong Hee and Park، نويسنده , , Myung Ok and Choi، نويسنده , , So Yoen and Kim، نويسنده , , Yong-Suk and Lee، نويسنده , , Seung Seok and Yoo، نويسنده , , Sun Dong and Lee، نويسنده , , Hye Suk and Lee، نويسنده , , Kang Choon، نويسنده ,
Abstract :
A capillary electrophoretic method (CE) was developed for the determination of the PEG-modification sites of three positional isomers of mono-PEG modified salmon calcitonins (mono-PEG-sCTs). Resistance to proteolytic degradation on the PEG modification sites resulted in different patterns of CE electropherograms for the tryptic digested mono-PEG-sCTs isomers, and the PEG modification sites were assigned accordingly. The PEG-modification sites were also confirmed directly by determining the molecular masses of the tryptic digested PEG-modified fragments of respective mono-PEG-sCT by the matrix-assisted laser desorption ionization time-of-flight (MALDI–TOF) mass spectrometry.
