Author/Authors :
Pastorello، نويسنده , , Elide A and Farioli، نويسنده , , Laura and Pravettoni، نويسنده , , Valerio and Giuffrida، نويسنده , , Maria Gabriella and Ortolani، نويسنده , , Claudio and Fortunato، نويسنده , , Donatella and Trambaioli، نويسنده , , Chiara and Scibola، نويسنده , , Elisabetta and Calamari، نويسنده , , Ambra M and Robino، نويسنده , , Anna M and Conti، نويسنده , , Amedeo، نويسنده ,
Abstract :
Background: Allergy to Prunoideae fruit (plum, peach, cherry and apricot) is one of the most frequent food allergies in southern Europe. All these fruits cross-react in vivo and in vitro, as they share their major allergen, a 9 kD lipid transfer protein (LTP). Objective: The aim of the study was the identification and molecular characterization of the major allergen of plum. Methods: The IgE pattern of reactivity to plums was investigated by SDS–PAGE and immunoblotting with the sera of 23 patients. The identified major allergen was purified by HPLC, using a cationic-exchange column followed by gel-filtration. Further characterization was achieved by periodic-Schiff stain, isoelectrofocusing and N-terminal amino acid sequencing. Results and conclusions: The major allergen of plum is a 9 kD lipid transfer protein, not glycosylated and with a basic character (pI>9), highly homologous to the major allergen of peach.
