Title of article
Determination of hydrogen sulfide and acid-labile sulfur in animal tissues by gas chromatography and ion chromatography
Author/Authors
Ubuka، نويسنده , , Toshihiko and Abe، نويسنده , , Tadashi and Kajikawa، نويسنده , , Rie and Morino، نويسنده , , Katsuya، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2001
Pages
7
From page
31
To page
37
Abstract
A sensitive and reliable method was developed for the determination of hydrogen sulfide and acid-labile sulfur (ALS) in animal tissues using gas chromatography with flame photometric detector (GC-FPD) and ion chromatography (IC). Hydrogen sulfide trapped in alkaline solution was determined by GC-FPD as hydrogen sulfide or by IC as sulfate after oxidation with hydrogen peroxide. Sodium sulfide used as a source of hydrogen sulfide was standardized by IC. Fresh rat liver and heart tissues contained 112.2±23.0 and 274.1±34.6 nmol/g of ALS respectively. Free hydrogen sulfide was not detected.
Keywords
Hydrogen sulphide , Acid-labile sulphur
Journal title
Journal of Chromatography B Biomedical Sciences and Applications
Serial Year
2001
Journal title
Journal of Chromatography B Biomedical Sciences and Applications
Record number
1705230
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