Determination of sameridine in blood plasma by nitrogen-selective gas chromatography

A gas chromatographic method was developed and validated for the determination of sameridine in human plasma. Sameridine is a new type of compound with both local anaesthetic and analgesic properties, when administered intrathecally. The method is based on liquid–liquid extraction of sameridine from 1.0 ml of plasma, followed by gas chromatography with nitrogen–phosphorus detection. Method validation results showed that this method is very sensitive, selective and robust. The limit of quantification was 1 nM for 1.0 ml of human plasma in the low-level range (1.00–75.0 nM) and the between-day accuracy and precision were measured at 99–104% of nominal values and 3.4–5.6% (RSD), respectively.