Title of article :
Development of sensitive high-performance liquid chromatography with fluorescence detection using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride as a labeling reagent for determination of bisphenol A in plasma samples
Author/Authors :
Watanabe، نويسنده , , Takaho and Yamamoto، نويسنده , , Hiroshi and Inoue، نويسنده , , Koichi and Yamaguchi، نويسنده , , Akiko and Yoshimura، نويسنده , , Yoshihiro and Kato، نويسنده , , Kayoko and Nakazawa، نويسنده , , Hiroyuki and Kuroda، نويسنده , , Naotaka and Nakashima، نويسنده , , Kenichiro، نويسنده ,
Abstract :
A sensitive HPLC method for determination of bisphenol A (BPA) in plasma samples using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a fluorescence labeling reagent was developed. The fluorescence labeling reaction was completed within 10 min at room temperature. DIB-Cl reacts with the phenolic hydroxyl group of BPA in the presence of triethylamine (TEA). The DIB-Cl derivative of BPA (DIB-BPA) was separated within 30 min with an ODS column using acetonitrile–water (90:10, v/v) as the isocratic eluent. Calibration graphs were linear over the range of 1.0–100 ng/ml (r=0.999). The detection limit of DIB-BPA was 0.05 ng/ml (2.5 pg) at a signal-to-noise ratio of 3. The relative standard deviations (RSDs) of the method for between-run were 1.0–5.0%. The analytical recoveries of known amounts (1.0 and 100 ng/ml) of BPA-spiked rabbit plasma were around 95%.
