Remarkable decrease in d-amino acid oxidase activity in the kidney of both prone and resistant strains of senescence-accelerated mouse

d-Amino acid oxidase (DAO) activity was remarkably low in the kidney of both prone P1 and resistant R1 strains of the senescence-accelerated mouse (SAM) compared with that of AKR/J mouse, in spite of the presence of a nearly equal amount of protein as demonstrated by Western blot analysis. A nucleotide sequence analysis of DAO-cDNA from SAMP1/Yag kidney indicated that the 541st guanine was replaced by adenine, resulting in the change of the 181st amino acid from glycine to arginine. The results of reverse transcription-polymerase chain reaction-restriction fragment length polymorphism analysis indicate the presence of this mutation in all SAM strains examined (P1, P2, P3 and R5).