Author/Authors :
Botta، نويسنده , , Bruno and Zappia، نويسنده , , Giovanni and Tafi، نويسنده , , Andrea and Botta، نويسنده , , Maurizio and Manetti، نويسنده , , Fabrizio and Cernia، نويسنده , , Enrico and Milana، نويسنده , , Giuliana and Palocci، نويسنده , , Cleofe and Soro، نويسنده , , Simonetta and Monache، نويسنده , , Giuliano Delle Monache، نويسنده ,
Abstract :
Immobilized lipase from Mucor miehei (RML) catalyzed the regioselective acylation of the C-2 side-chain of the C-alkyl resorcin[4]arene tetra-alcohol 1 in the 1,2-alternate form in organic solvents using vinyl acetate as acylating reagent. The influence of reaction parameters and solvent choice were also studied. Docking simulations allowed the determination of the binding geometry of 1, revealing the importance of Trp88 residue in stabilizing the Michaelis–Menten complex between enzyme and substrate.
Keywords :
resorcinarene , Transesterification , molecular modeling , Docking , Lipase
