Author/Authors :
Kosjek، نويسنده , , B. and Stampfer، نويسنده , , W. and Glueck، نويسنده , , S.M. and Pogorevc، نويسنده , , M. and Ellmer، نويسنده , , U. and Wallner، نويسنده , , S.R. and Koegl، نويسنده , , M.F. and Poessl، نويسنده , , T.M. and Mayer، نويسنده , , S.F. and Ueberbacher، نويسنده , , B. and Faber، نويسنده , , K. and Kroutil، نويسنده , , W.، نويسنده ,
Abstract :
The organic solvent-stable redox-system of Rhodococcus ruber DSM 44541, which allows the efficient oxidation/reduction of sec-alcohols/ketones at the expense of acetone/2-propanol, respectively, as cosubstrate was optimized with respect to a maximum of alcohol dehydrogenase activity during cell growth. Comparison of the fermentation of R. ruber DSM 44541 in shake flasks cultures (1 l flask with 250 ml medium) and in a bioreactor (15 l with 10 l working volume) revealed that the desired organic solvent-stable alcohol dehydrogenase activity reached its maximum during the log phase for the bioreactor. In contrast, in shake flasks the maximum of activity was reached during the stationary phase.
Keywords :
Fermentation , Activity-growth , sec-Alcohol dehydrogenase , Rhodococcus ruber
