Title of article
Improvement of thermostability of cold-active serine alkaline protease from the psychrotrophic bacterium Shewanella sp. strain Ac10 by rational mutagenesis
Author/Authors
Kulakova، نويسنده , , Ljudmila and Galkin، نويسنده , , Andrey and Nakayama، نويسنده , , Toru and Nishino، نويسنده , , Tokuzo and Esaki، نويسنده , , Nobuyoshi، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2003
Pages
5
From page
113
To page
117
Abstract
A serine alkaline protease (SapSh) from a psychrophilic bacterium Shewanella sp. strain Ac10 is a cold-active subtilase with low thermostability [Appl. Environ. Microbiol. 65 (1999) 611–617]. By means of homology modeling with other subtilase structures, we have constructed a mutant SapSh containing an extra salt bridge on its surface that exhibits higher thermostability and even higher Vmax/Km, app value than those of the wild-type SapSh.
Keywords
Cold-active enzymes , cold-adapted enzymes , thermostability , homology modeling , protease , psychrophile
Journal title
Journal of Molecular Catalysis B Enzymatic
Serial Year
2003
Journal title
Journal of Molecular Catalysis B Enzymatic
Record number
1709661
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