Author/Authors :
Rahimi، نويسنده , , Hassan and Soro، نويسنده , , Simonetta and Rughetti، نويسنده , , Aurelia and Palocci، نويسنده , , Cleofe and Biffoni، نويسنده , , Mauro and Barachini، نويسنده , , Serena and Taurino، نويسنده , , Federica and Cernia، نويسنده , , Enrico and Frati، نويسنده , , Luigi and Nuti، نويسنده , , Marianna، نويسنده ,
Abstract :
We generated monoclonal antibodies (MoAbs) BF11 and VNH9 against Candida rugosa lipase (CRL), one of the most widely used lipolytic enzymes. The antibodies, both of the IgG1 isotype recognize specie specific and distinct antigenic determinants shared by different CRL isoforms present both in the native and in the denaturated form of the enzyme. The CRL enzyme maintained its enzymatic activity even when immunocomplexed with MoAb BF11 and VNH9. These novel reagents are of interest for their possible application as immobilization and purification systems, and during fermentation processes.
