Author/Authors :
Radka Snajdrova، نويسنده , , Radka and Kristov?-Mylerov?، نويسنده , , Veronika and Crestia، نويسنده , , Dominique and Nikolaou، نويسنده , , Konstantina and Kuzma، نويسنده , , Marek and Lemaire، نويسنده , , Marielle and Gallienne، نويسنده , , Estelle and Bolte، نويسنده , , Jean and Bezou?ka، نويسنده , , Karel and K?en، نويسنده , , Vladim??r and Mart??nkov?، نويسنده , , Ludmila، نويسنده ,
Abstract :
A nitrile-converting enzyme activity was induced in Aspergillus niger K10 by 3-cyanopyridine. The whole cell biocatalyst was active at pH 3–11 and hydrolyzed the cyano group into acid and/or amide functions in benzonitrile as well as in its meta- and para-substituted derivatives, cyanopyridines, 2-phenylacetonitrile and thiophen-2-acetonitrile. Amides constituted a significant part of the total biotransformation products of 2- and 4-cyanopyridine, 4-chlorobenzonitrile, 4-tolunitrile and 1,4-dicyanobenzene, while α-substituted acrylonitriles gave amides as the sole products.
Keywords :
Nitrilase , amidase , Aspergillus niger , Nitrile hydratase , Nitrile-converting enzymes
