Bio-gas production by co-fermentation from the brown algae, Laminaria japonica

In this study, the optimization of bio-gas produced from Laminaria japonica through co-fermentation using hydrolysis and bio-gas production microorganisms was investigated. Bio-gas production was increased by using a mixed culture with Clostridium butyricum and Erwinia tasmaniensis, and the total hydrogen and methane levels were 327.47% and 354.99% higher, respectively. When lower oxygen contents were used in the flask, the bio-gas production yield increased. The optimual E/C ratio was determined to be 1/1, and the hydrogen and methane gas production levels under these conditions were 356.03 mL/L and 2243.59 mL/L, respectively.