Title of article
A metal ion-based method for the screening of nitrilases
Author/Authors
Yazbeck، نويسنده , , Daniel R. and Durao، نويسنده , , Paulo J. and Xie، نويسنده , , Zhiyi and Tao، نويسنده , , Junhua، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
4
From page
156
To page
159
Abstract
In this paper we describe a colorimetric method for the screening of nitrilases. When a buffered solution of CoCl2 is added to a nitrilase-catalyzed hydrolysis reaction, the ammonia product forms a complex with the cobalt ion resulting in a color change from light pink to yellow, which can readily be quantified using a spectrophotometer at 375 nm. This method has been demonstrated for both wild-type and evolved nitrilases.
Keywords
Enantioselectivity , UV–vis spectroscopy , high-throughput screening , Nitrilase
Journal title
Journal of Molecular Catalysis B Enzymatic
Serial Year
2006
Journal title
Journal of Molecular Catalysis B Enzymatic
Record number
1710872
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