Electro-oxidation and determination of gemcitabine hydrochloride, an anticancer drug at gold electrode

Electrochemical behavior of an anticancer drug, gemcitabine hydrochloride (GMB) was studied in 10.4 pH with 0.2 M phosphate buffer solution as supporting electrolyte at 25 ± 0.1 °C at gold electrode (GE) using different voltammetric techniques. The electrochemical process was observed to be diffusion controlled, irreversible and involving one-electron oxidation. Under optimal conditions, the peak current was proportional to GMB concentration in the range of 0.1–15 μM with a detection limit of 0.06 μM by differential pulse voltammetry. The method was developed for the determination of GMB in pharmaceutical formulations and urine as a real sample.