Silane graft copolymer modified with sulphonic acid functional groups used for immobilization of trypsin from aqueous solutions via adsorption process

A novel silane based adsorbent material, [stearyl alcohol (SA)-grafted-epichlorohydrin (E)]-grafted-aminoproypyl silanetriol (APST) was synthesized and functionalized with sulfonic acid [(SA-g-E)-g-APST/SO3H]. The adsorbent material was characterized by FTIR, XRD, SEM and BET analysis. Protein–particle interaction was studied by the immobilization of trypsin (TRY) using batch adsorption process. Maximum adsorption was attained at pH 7.0. Adsorption process followed pseudo-second-order kinetics; film diffusion controlled and Sips isotherm mechanism. Evaluation of separation factor (RL) and thermodynamic parameters revealed the favorability of adsorption process. Adsorption/desorption studies, for over six cycles showed the repeatability and regeneration capability of the adsorbent.