Glucose oxidase and catalase co-immobilization on biosynthesized nanoporous SiO2 for removal of dissolved oxygen in water: Corrosion controlling of boilers

Nanoporous SiO2 was synthesized by sol–gel method using dry yeast cells as a template. Additionally, characterization of the biosynthesized nanoporous SiO2 was investigated. Bio-synthesized nanoporous SiO2 has the specific surface area of 460 m2/g and total pore volume of 0.36 cm3/g. Co-immobilization of glucose oxidase and catalase by adsorption method was done on biosynthesized nanoporous SiO2 and immobilized enzymes were used for removal of water dissolved oxygen. Additionally, modeling and optimization for removal of water dissolved oxygen was performed using rotatable response surface methodology (RSM). Experimental values were in good agreement with the predicated ones and the model was highly significant.