One-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-d-ribose

The one-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-d-ribose (5-FDR) from ATP and l-methionine using S-adenosyl-l-methionine synthase (MetK), fluorinase and methylthioadenosine nucleosidase (MtnN) in the presence of fluoride ion, was described. Especially, for the purpose of the sustainable development of fluorine chemistry, the reuse of fluoride ion generated from BF4 ionic liquids and/or the biodegradation of BTF in the one-pot synthetic process to 5-FDR, was described.