Effect of linoleic acid induction on the production of 1-octen-3-ol by the lipoxygenase and hydroperoxide lyase activities of Penicillium camemberti

The effect of induction by linoleic acid on the biomass production, glucose consumption, change in pH values and 1-octen-3-ol profile were investigated during the growth of Penicillium camemberti. The results showed that the presence of 1 g of linoleic acid per liter of culture medium increased the biomass from 3.0 to 7.0 g/l. In addition, incubation of crude enzymatic extracts, obtained from induced and non-induced P. camemberti cultures, indicated that the amount of 1-octen-3-ol produced by the induced enzymatic extract was approximately twice compared to that obtained by the non-induced one. The presence of 0.5 g linoleic acid in the culture medium resulted in the generation of 1.8 μg 1-octen-3-ol per mg protein as opposed to 0.8 μg 1-octen-3-ol per mg protein obtained using 1.0 mg linoleic acid. Moreover, the bioconversion of the 10-hydroperoxide of linoleic acid into 1-octen-3-ol by the enzymatic extract obtained from the induced microbial biomass was twice as high as that obtained with the non-induced one, corresponding to 9.0 and 4.6 μg 1-octen-3-ol per mg protein, respectively.