High-yield enzymatic synthesis of O-allyl β-d-galactopyranoside

A high-yield synthesis of O-allyl β-d-galactopyranoside was carried out by the use of Aspergillus oryzae β-galactosidase. The reaction was carried out employing p-nitrophenyl β-d-galactopyranoside as the donor and a large excess of allyl alcohol as the acceptor. The molar yield was 65.6%, corresponding to an improvement of 41.3% with respect to the best results previously reported with other systems, and of 80.2% with respect to the results obtained using the same enzyme.