Author/Authors :
Shimada، نويسنده , , Hideaki and Fujiki، نويسنده , , Sumie and Oginuma، نويسنده , , Michiko and Asakawa، نويسنده , , Makio and Okawara، نويسنده , , Tadashi and Kato، نويسنده , , Keisuke and Yamamura، نويسنده , , Shigeo and Akita، نويسنده , , Hiroyuki and Hara، نويسنده , , Akira and Imamura، نويسنده , , Yorishige and Hara، نويسنده ,
Abstract :
Optically active (−)- and (+)-α-phenyl-4-pyridylmethanol [(−)- and (+)-PPOL] were synthesized from 4-benzoylpyridine (4-BP) and established to possess (S)- and (R)-configurations, respectively, by X-ray single crystal structure analysis. When 4-BP was incubated in the reaction mixture containing recombinant pig heart carbonyl reductase (rPHCR) and NADPH, the reduction product appeared as a main peak corresponding to (S)-(−)-PPOL on high-performance liquid chromatography (HPLC). This result indicates that rPHCR has the ability to reduce stereoselectively 4-BP to (S)-(−)-PPOL with high optical purity.
Keywords :
stereoselective reduction , 4-Benzoylpyridine , Recombinant enzyme , Optically active alcohol metabolite , Carbonyl reductase
